• The Coordinated Action of MYB Activators and Repressors Controls Proanthocyanidin and Anthocyanin Biosynthesis in Vaccinium 

      Lafferty, Declan J.; Espley, Richard V.; Deng, Cecilia H; Dare, Andrew P.; Günther, Catrin S.; Jaakola, Laura; Karppinen, Katja; Boase, Murray R.; Wang, Lei; Luo, Henry; Allan, Andrew C.; Albert, Nick W. (Journal article; Tidsskriftartikkel; Peer reviewed, 2022-06-24)
      Vaccinium berries are regarded as “superfoods” owing to their high concentrations of anthocyanins, flavonoid metabolites that provide pigmentation and positively affect human health. Anthocyanin localization differs between the fruit of cultivated highbush blueberry (V. corymbosum) and wild bilberry (V. myrtillus), with the latter having deep red flesh coloration. Analysis of comparative ...
    • Flavonoid biosynthesis is differentially altered in detached and attached ripening bilberries in response to spectral light quality 

      Samkumar, Amos; Karppinen, Katja; McGhie, Tony K.; Espley, Richard V.; Martinussen, Inger; Jaakola, Laura (Journal article; Tidsskriftartikkel; Peer reviewed, 2022-07-22)
      Light spectral quality is known to affect flavonoid biosynthesis during fruit ripening. However, the response of fruits to different light conditions, when ripening autonomously from the parent plant (detached), has been less explored. In this study, we analyzed the effect of light quality on detached and naturally ripening (attached) non-climacteric wild bilberry (Vaccinium myrtillus L.) fruits ...
    • MYBA and MYBPA transcription factors co-regulate anthocyanin biosynthesis in blue-coloured berries 

      Karppinen, Katja; Lafferty, Declan J.; Albert, Nick W.; Mikkola, Nelli; McGhie, Tony K.; Allan, Andrew C.; Afzal, Bilal M.; Häggman, Hely; Espley, Richard V.; Jaakola, Laura (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-08-05)
      <p>We used blue berries of bilberry (Vaccinium myrtillus) to comprehensively characterise flavonoid-regulating R2R3 MYBs, which revealed a new type of co-regulation in anthocyanin biosynthesis between members of MYBA-, MYBPA1- and MYBPA2-subgroups. <p>VmMYBA1, VmMYBPA1.1 and VmMYBPA2.2 expression was elevated at berry ripening and by abscisic acid treatment. Additionally, VmMYBA1 and VmMYBPA1.1 ...
    • Red and blue light treatments of ripening bilberry fruits reveal differences in signalling through abscisic acid-regulated anthocyanin biosynthesis 

      Samkumar, Amos; Jones, Dan; Karppinen, Katja; Dare, Andrew P.; Sipari, Nina; Espley, Richard V.; Martinussen, Inger; Jaakola, Laura (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-08-01)
      The biosynthesis of anthocyanins has been shown to be influenced by light quality. However, the molecular mechanisms underlying the light-mediated regulation of fruit anthocyanin biosynthesis are not well understood. In this study, we analysed the effects of supplemental red and blue light on the anthocyanin biosynthesis in non-climacteric bilberry (<i>Vaccinium myrtillus</i> L.). After 6 days of ...
    • Spatiotemporal modulation of flavonoid metabolism in blueberries 

      Günther, Catrin Sonja; Dare, Andrew P.; McGhie, Tony K.; Deng, Cecilia; Lafferty, Declan J.; Plunkett, Blue J.; Grierson, Ella R.P.; Turner, Janice L.; Jaakola, Laura; Albert, Nick W.; Espley, Richard V. (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-05-13)
      Blueberries are distinguished by their purple-blue fruit color, which develops during ripening and is derived from a characteristic composition of flavonoid-derived anthocyanin pigments. The production of anthocyanins is confined to fruit skin, leaving the colorless fruit flesh devoid of these compounds. By linking accumulation patterns of phenolic metabolites with gene transcription in Northern ...